"study of antiamoebic antibody in amoebiasis using elisa and rid techniques"
نویسندگان
چکیده
to study the humoral immune response to entamoeba histolytica infection, sera prepared from different cases of amoebiasis from gastroenterology ward of b.h.u hospital, including15 cases with amoebic liver abscess (ala), 10 cases with acute amoebic dysentery (ad) and 10 controls were examined by enzyme linked immuno sorbent assay (elisa), using crude amoebic antigen prepared from entamoeba histolytica nih: 200 grown axenically and its three chromatographed fractions, i.e.fraction i (fi), fraction ii (fii) and fraction iii (fiii). efficacies of different antigens in detecting anti amoebic antibodies were compared. estimation of immunoglobulin g (igg), immunoglobulin m (igm) and immunoglobulin a (iga) levels were also carried out employing single radial immuno diffusion (rid) technique. the results indicated that crude amoebic antigen and fraction i (fi) were more efficient in antibody detection while, potencies of fraction ii (fii) and fraction iii (fiii) were lower as antigens for serodiagnoses. on estimating different immunoglobulin levels, it was found out that there was significant increase in igg level in amoebiasis group while, no significant increase was observed in iga level. igm level was increased only in amoebic liver abscess cases compared to negative controls.
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15 صفحه اولserological diagnosis of amoebiasis by elisa in human
introduction: determination of the efficacy of crude entamoeba histolytica extract and its fractions (fi,fii & fiii) in antiamoebic antibody detection by elisa. material and methods: entamoeba histolytica nih:200 was cultured axenically and different fractionsof its crude amoebic extract, i.e. fi, fii & fiii, were obtained by column chromatography. efficacy of crudeand fractioned antigens for a...
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عنوان ژورنال:
iranian journal of public healthجلد ۳۲، شماره ۴، صفحات ۱۳-۱۸
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